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bay 61 3606 hcl  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology bay 61 3606 hcl
    Bay 61 3606 Hcl, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/syk+inhibitor+iv+bay/Syk+Inhibitor+IV%2C+BAY+61-3606+HCl/pmc12534707-35-3-9
    Average 92 stars, based on 8 article reviews
    bay 61 3606 hcl - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Infection:

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection.
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol® reagent was purchased September 2021 | Volume 12 | Article 747370 from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol ® reagent was purchased from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Activation Assay:

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection.
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol® reagent was purchased September 2021 | Volume 12 | Article 747370 from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol ® reagent was purchased from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Concentration Assay:

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection.
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol® reagent was purchased September 2021 | Volume 12 | Article 747370 from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol ® reagent was purchased from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Western Blot:

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection.
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol® reagent was purchased September 2021 | Volume 12 | Article 747370 from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol ® reagent was purchased from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Expressing:

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection.
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol® reagent was purchased September 2021 | Volume 12 | Article 747370 from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol ® reagent was purchased from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection.
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol® reagent was purchased September 2021 | Volume 12 | Article 747370 from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol ® reagent was purchased from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Enzyme-linked Immunosorbent Assay:

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection.
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol® reagent was purchased September 2021 | Volume 12 | Article 747370 from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol ® reagent was purchased from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Microscopy:

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection.
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol® reagent was purchased September 2021 | Volume 12 | Article 747370 from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol ® reagent was purchased from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Control:

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection.
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol® reagent was purchased September 2021 | Volume 12 | Article 747370 from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol ® reagent was purchased from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Fluorescence:

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection.
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol® reagent was purchased September 2021 | Volume 12 | Article 747370 from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection
    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol ® reagent was purchased from Invitrogen Thermo Fisher Scientific, Inc.. The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).The H&E stain kit (cat. no. G1120), RIPA buffer (cat. no. R0020), DAPI solution (cat. no. C0060) and 4% paraformaldehyde (cat. no. P1110) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).



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    FIGURE 6 | Phosphorylated Syk was involved in S. aureus infection-induced NEK7-NLRP3 signaling pathway activation. J774A.1 cells (A–I) and PECs (J–M) pretreated with or without <t>Syk</t> <t>inhibitor</t> <t>(BAY</t> 613606) at the final concentration of 1 mmol/L for 2 h followed by S. aureus infecton (MOI of 1 or 5) for different time. (A) The protein levels of t-Syk and p-Syk were detected by western blot and analyzed by relative densitometric quantification followed by S. aureus infection (MOI of 5) for 30 min in J774A.1 cells and PECs (J). (B) The mRNA expression levels of caspase-1 and Il-1b were quantified by RT-PCR. (C) The protein expression levels of caspase-1, p20, pro-IL-1b and mIL-1b were detected by western blot and analyzed by relative densitometric quantification. (D) The supernatants were collected at 48 h after S aureus infection. IL-1b and IL-18 in the supernatants were determined by ELISA in J774A.1 cells and PECs (M). (E) The ASC specks formation were imaged via fluorescence microscopy. (Scale bar: 100 µm.). (F) ASC specks were quantitied in more than 200 cells from at least 5 different fields. (G) The mRNA expression level of nek7 was examined by RT-PCR. (H) The protein expression level of NEK7 was detected by western blot and analyzed by relative densitometric quantification in J774A.1 cells and PECs (K). (I) The NEK7-NLRP3 compound were analysed by coimmunoprecipitation in J774A.1 cells and PECs (L). Similar results were obtained in three independent experiments in data. Data shown are means ± SEMs; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. non- infection control group, #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 vs. infection control group.
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    FIGURE 6 | Phosphorylated Syk was involved in S. aureus infection-induced NEK7-NLRP3 signaling pathway activation. J774A.1 cells (A–I) and PECs (J–M) pretreated with or without <t>Syk</t> <t>inhibitor</t> <t>(BAY</t> 613606) at the final concentration of 1 mmol/L for 2 h followed by S. aureus infecton (MOI of 1 or 5) for different time. (A) The protein levels of t-Syk and p-Syk were detected by western blot and analyzed by relative densitometric quantification followed by S. aureus infection (MOI of 5) for 30 min in J774A.1 cells and PECs (J). (B) The mRNA expression levels of caspase-1 and Il-1b were quantified by RT-PCR. (C) The protein expression levels of caspase-1, p20, pro-IL-1b and mIL-1b were detected by western blot and analyzed by relative densitometric quantification. (D) The supernatants were collected at 48 h after S aureus infection. IL-1b and IL-18 in the supernatants were determined by ELISA in J774A.1 cells and PECs (M). (E) The ASC specks formation were imaged via fluorescence microscopy. (Scale bar: 100 µm.). (F) ASC specks were quantitied in more than 200 cells from at least 5 different fields. (G) The mRNA expression level of nek7 was examined by RT-PCR. (H) The protein expression level of NEK7 was detected by western blot and analyzed by relative densitometric quantification in J774A.1 cells and PECs (K). (I) The NEK7-NLRP3 compound were analysed by coimmunoprecipitation in J774A.1 cells and PECs (L). Similar results were obtained in three independent experiments in data. Data shown are means ± SEMs; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. non- infection control group, #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 vs. infection control group.
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    FIGURE 6 | Phosphorylated Syk was involved in S. aureus infection-induced NEK7-NLRP3 signaling pathway activation. J774A.1 cells (A–I) and PECs (J–M) pretreated with or without <t>Syk</t> <t>inhibitor</t> <t>(BAY</t> 613606) at the final concentration of 1 mmol/L for 2 h followed by S. aureus infecton (MOI of 1 or 5) for different time. (A) The protein levels of t-Syk and p-Syk were detected by western blot and analyzed by relative densitometric quantification followed by S. aureus infection (MOI of 5) for 30 min in J774A.1 cells and PECs (J). (B) The mRNA expression levels of caspase-1 and Il-1b were quantified by RT-PCR. (C) The protein expression levels of caspase-1, p20, pro-IL-1b and mIL-1b were detected by western blot and analyzed by relative densitometric quantification. (D) The supernatants were collected at 48 h after S aureus infection. IL-1b and IL-18 in the supernatants were determined by ELISA in J774A.1 cells and PECs (M). (E) The ASC specks formation were imaged via fluorescence microscopy. (Scale bar: 100 µm.). (F) ASC specks were quantitied in more than 200 cells from at least 5 different fields. (G) The mRNA expression level of nek7 was examined by RT-PCR. (H) The protein expression level of NEK7 was detected by western blot and analyzed by relative densitometric quantification in J774A.1 cells and PECs (K). (I) The NEK7-NLRP3 compound were analysed by coimmunoprecipitation in J774A.1 cells and PECs (L). Similar results were obtained in three independent experiments in data. Data shown are means ± SEMs; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. non- infection control group, #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 vs. infection control group.
    Syk Kinase Inhibitor Iv Bay 61 3606, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    FIGURE 6 | Phosphorylated Syk was involved in S. aureus infection-induced NEK7-NLRP3 signaling pathway activation. J774A.1 cells (A–I) and PECs (J–M) pretreated with or without <t>Syk</t> <t>inhibitor</t> <t>(BAY</t> 613606) at the final concentration of 1 mmol/L for 2 h followed by S. aureus infecton (MOI of 1 or 5) for different time. (A) The protein levels of t-Syk and p-Syk were detected by western blot and analyzed by relative densitometric quantification followed by S. aureus infection (MOI of 5) for 30 min in J774A.1 cells and PECs (J). (B) The mRNA expression levels of caspase-1 and Il-1b were quantified by RT-PCR. (C) The protein expression levels of caspase-1, p20, pro-IL-1b and mIL-1b were detected by western blot and analyzed by relative densitometric quantification. (D) The supernatants were collected at 48 h after S aureus infection. IL-1b and IL-18 in the supernatants were determined by ELISA in J774A.1 cells and PECs (M). (E) The ASC specks formation were imaged via fluorescence microscopy. (Scale bar: 100 µm.). (F) ASC specks were quantitied in more than 200 cells from at least 5 different fields. (G) The mRNA expression level of nek7 was examined by RT-PCR. (H) The protein expression level of NEK7 was detected by western blot and analyzed by relative densitometric quantification in J774A.1 cells and PECs (K). (I) The NEK7-NLRP3 compound were analysed by coimmunoprecipitation in J774A.1 cells and PECs (L). Similar results were obtained in three independent experiments in data. Data shown are means ± SEMs; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. non- infection control group, #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 vs. infection control group.
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    FIGURE 6 | Phosphorylated Syk was involved in S. aureus infection-induced NEK7-NLRP3 signaling pathway activation. J774A.1 cells (A–I) and PECs (J–M) pretreated with or without <t>Syk</t> <t>inhibitor</t> <t>(BAY</t> 613606) at the final concentration of 1 mmol/L for 2 h followed by S. aureus infecton (MOI of 1 or 5) for different time. (A) The protein levels of t-Syk and p-Syk were detected by western blot and analyzed by relative densitometric quantification followed by S. aureus infection (MOI of 5) for 30 min in J774A.1 cells and PECs (J). (B) The mRNA expression levels of caspase-1 and Il-1b were quantified by RT-PCR. (C) The protein expression levels of caspase-1, p20, pro-IL-1b and mIL-1b were detected by western blot and analyzed by relative densitometric quantification. (D) The supernatants were collected at 48 h after S aureus infection. IL-1b and IL-18 in the supernatants were determined by ELISA in J774A.1 cells and PECs (M). (E) The ASC specks formation were imaged via fluorescence microscopy. (Scale bar: 100 µm.). (F) ASC specks were quantitied in more than 200 cells from at least 5 different fields. (G) The mRNA expression level of nek7 was examined by RT-PCR. (H) The protein expression level of NEK7 was detected by western blot and analyzed by relative densitometric quantification in J774A.1 cells and PECs (K). (I) The NEK7-NLRP3 compound were analysed by coimmunoprecipitation in J774A.1 cells and PECs (L). Similar results were obtained in three independent experiments in data. Data shown are means ± SEMs; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. non- infection control group, #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 vs. infection control group.
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    Image Search Results


    FIGURE 6 | Phosphorylated Syk was involved in S. aureus infection-induced NEK7-NLRP3 signaling pathway activation. J774A.1 cells (A–I) and PECs (J–M) pretreated with or without Syk inhibitor (BAY 613606) at the final concentration of 1 mmol/L for 2 h followed by S. aureus infecton (MOI of 1 or 5) for different time. (A) The protein levels of t-Syk and p-Syk were detected by western blot and analyzed by relative densitometric quantification followed by S. aureus infection (MOI of 5) for 30 min in J774A.1 cells and PECs (J). (B) The mRNA expression levels of caspase-1 and Il-1b were quantified by RT-PCR. (C) The protein expression levels of caspase-1, p20, pro-IL-1b and mIL-1b were detected by western blot and analyzed by relative densitometric quantification. (D) The supernatants were collected at 48 h after S aureus infection. IL-1b and IL-18 in the supernatants were determined by ELISA in J774A.1 cells and PECs (M). (E) The ASC specks formation were imaged via fluorescence microscopy. (Scale bar: 100 µm.). (F) ASC specks were quantitied in more than 200 cells from at least 5 different fields. (G) The mRNA expression level of nek7 was examined by RT-PCR. (H) The protein expression level of NEK7 was detected by western blot and analyzed by relative densitometric quantification in J774A.1 cells and PECs (K). (I) The NEK7-NLRP3 compound were analysed by coimmunoprecipitation in J774A.1 cells and PECs (L). Similar results were obtained in three independent experiments in data. Data shown are means ± SEMs; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. non- infection control group, #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 vs. infection control group.

    Journal: Frontiers in immunology

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection.

    doi: 10.3389/fimmu.2021.747370

    Figure Lengend Snippet: FIGURE 6 | Phosphorylated Syk was involved in S. aureus infection-induced NEK7-NLRP3 signaling pathway activation. J774A.1 cells (A–I) and PECs (J–M) pretreated with or without Syk inhibitor (BAY 613606) at the final concentration of 1 mmol/L for 2 h followed by S. aureus infecton (MOI of 1 or 5) for different time. (A) The protein levels of t-Syk and p-Syk were detected by western blot and analyzed by relative densitometric quantification followed by S. aureus infection (MOI of 5) for 30 min in J774A.1 cells and PECs (J). (B) The mRNA expression levels of caspase-1 and Il-1b were quantified by RT-PCR. (C) The protein expression levels of caspase-1, p20, pro-IL-1b and mIL-1b were detected by western blot and analyzed by relative densitometric quantification. (D) The supernatants were collected at 48 h after S aureus infection. IL-1b and IL-18 in the supernatants were determined by ELISA in J774A.1 cells and PECs (M). (E) The ASC specks formation were imaged via fluorescence microscopy. (Scale bar: 100 µm.). (F) ASC specks were quantitied in more than 200 cells from at least 5 different fields. (G) The mRNA expression level of nek7 was examined by RT-PCR. (H) The protein expression level of NEK7 was detected by western blot and analyzed by relative densitometric quantification in J774A.1 cells and PECs (K). (I) The NEK7-NLRP3 compound were analysed by coimmunoprecipitation in J774A.1 cells and PECs (L). Similar results were obtained in three independent experiments in data. Data shown are means ± SEMs; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. non- infection control group, #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 vs. infection control group.

    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol® reagent was purchased September 2021 | Volume 12 | Article 747370 from Invitrogen Thermo Fisher Scientific, Inc.

    Techniques: Infection, Activation Assay, Concentration Assay, Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Microscopy, Control

    FIGURE 7 | Syk/JNK signaling was critical for activation of the NLRP3 inflammasome upon S. aureus infection. Macrophages were infected with S. aureus (MOI of 5) for 2 h by pretreated with or without Syk-siRNA (A, B) for 48 h or Syk inhibitor (BAY) (C–E) for 2h. (A) The protein expression level of p-JNK was detected by western blot and analyzed by relative densitometric quantification in J774A.1 cells. (B) The mRNA expression level of jnk was quantified by RT-PCR. (C)The protein expression level of p-JNK was detected by western blot and analyzed by relative densitometric quantification in J774A.1 cells and PECs (E). (D) The mRNA expression level of jnk was quantified by RT-PCR. Similar results were obtained in three independent experiments in data. Data shown are means ± SEMs; **P < 0.01, ****P < 0.0001 vs. non-infection control group, #P < 0.05, ###P < 0.001, ####P < 0.0001 vs. infection control group.

    Journal: Frontiers in immunology

    Article Title: NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection.

    doi: 10.3389/fimmu.2021.747370

    Figure Lengend Snippet: FIGURE 7 | Syk/JNK signaling was critical for activation of the NLRP3 inflammasome upon S. aureus infection. Macrophages were infected with S. aureus (MOI of 5) for 2 h by pretreated with or without Syk-siRNA (A, B) for 48 h or Syk inhibitor (BAY) (C–E) for 2h. (A) The protein expression level of p-JNK was detected by western blot and analyzed by relative densitometric quantification in J774A.1 cells. (B) The mRNA expression level of jnk was quantified by RT-PCR. (C)The protein expression level of p-JNK was detected by western blot and analyzed by relative densitometric quantification in J774A.1 cells and PECs (E). (D) The mRNA expression level of jnk was quantified by RT-PCR. Similar results were obtained in three independent experiments in data. Data shown are means ± SEMs; **P < 0.01, ****P < 0.0001 vs. non-infection control group, #P < 0.05, ###P < 0.001, ####P < 0.0001 vs. infection control group.

    Article Snippet: The JNK inhibitor II SP600125 (SP) was purchased from Merck KGaA, Inc. Syk inhibitor IV BAY 61-3606 was purchased from Santa Cruz Biotechnology, Inc. TRIzol® reagent was purchased September 2021 | Volume 12 | Article 747370 from Invitrogen Thermo Fisher Scientific, Inc.

    Techniques: Activation Assay, Infection, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Control